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肝素酶(HPSE)多克隆抗體

Polyclonal Antibody to Heparanase (HPSE)

HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;

  • 肝素酶(HPSE)多克隆抗體 產品包裝(模擬)
  • 肝素酶(HPSE)多克隆抗體 產品包裝(模擬)
  • 肝素酶(HPSE)多克隆抗體 DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 肝素酶(HPSE)多克隆抗體 DAB staining on IHC-P;
    Sample: Porcine Spleen Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human HPSE Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 肝素酶(HPSE)多克隆抗體 DAB staining on IHC-P;
    Sample: Human Spleen Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 肝素酶(HPSE)多克隆抗體 FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • 肝素酶(HPSE)多克隆抗體 FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20μg/ml Rabbit Anti-Human HPSE Antibody
    Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA711Hu02.jpg Figure. Western Blot; Sample: Recombinant HPA, Human.
  • 肝素酶(HPSE)多克隆抗體 Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: HepG2 cell lysate; Lane3: Jurkat cell lysate;
    Primary Ab: 0.1μg/ml Rabbit Anti-Human HPSE Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 肝素酶(HPSE)多克隆抗體 Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: HPA knockout HepG2 cell lysate;
    Predicted MW: 43,53,55,61kd
    Observed MW: 68kd
    Primary Ab: 1μg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

該抗體是針對HPSE的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HPSE。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩定性

熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。

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編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
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參考文獻

雜志 參考文獻
Plos one PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source]
Journal of Medical Science Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source]
Int J Clin Exp Pathol Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183]
Tumour Biology PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733]
Journal of Medical Science Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84]
Renal Failure Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624]
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