血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay)
ANG1; AGP1; AGPT; ANG-I
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA008Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.01-15ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 86-103 | 98 |
| heparin plasma(n=5) | 82-96 | 90 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-91% | 93-101% | 99-105% | 93-104% |
| EDTA plasma(n=5) | 86-99% | 88-102% | 86-101% | 78-102% |
| heparin plasma(n=5) | 87-97% | 84-91% | 78-89% | 79-98% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合，然后加入生物素化的血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法)呈正相關。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA008Hu01 | 血管生成素1(ANGPT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA008Hu02 | 血管生成素1(ANGPT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA008Hu03 | 血管生成素1(ANGPT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA008Hu01 | 血管生成素1(ANGPT1)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA008Hu02 | 血管生成素1(ANGPT1)多克隆抗體 | WB |
| UFAA008Hu01 | 抗血管生成素1(ANGPT1)多克隆抗體 | FCM |
| ULAA008Hu71 | 血管生成素1(ANGPT1)多克隆抗體(生物素標記) | WB |
| UMAA008Hu23 | 血管生成素1(ANGPT1)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA008Hu24 | 血管生成素1(ANGPT1)單克隆抗體 | ICC/IF |
| UMAA008Hu21 | 血管生成素1(ANGPT1)單克隆抗體 | WB |
| UMAA008Hu25 | 血管生成素1(ANGPT1)單克隆抗體 | ICC/IF |
| UMAA008Hu22 | 血管生成素1(ANGPT1)單克隆抗體 | WB |
| UFAA008Hu04 | 抗血管生成素1(ANGPT1)單克隆抗體 | FCM |
| UFAA008Hu02 | 抗血管生成素1(ANGPT1)單克隆抗體 | FCM |
| UFAA008Hu08 | 抗血管生成素1(ANGPT1)單克隆抗體 | FCM |
| UFAA008Hu06 | 抗血管生成素1(ANGPT1)單克隆抗體 | FCM |
| ULAA008Hu72 | 血管生成素1(ANGPT1)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA008Hu | 血管生成素1(ANGPT1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA008Hu | 血管生成素1(ANGPT1)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA008Hu | 血管生成素1(ANGPT1)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA008Hu | 血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| J Atheroscler Thromb | High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
| Am J Transl Res | Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310] |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19] |
| Turkish Journal of Medicl Sciences | A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270] |
| Sci Rep.? | Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491] |
| PeerJ? | The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715] |
| Molecular&cellular proteomics | Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167] |
| Tumor Biology | Cervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059] |
| Free Patents online | RHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884] |
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| Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214] | |
| BIOLOGY?OF?REPRODUCTION | Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827] |
| Biochem Biophys Res Commun | Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538] |
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| Yonsei Med J | RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway [Pubmed:35619579] |
