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27kDa熱休克蛋白(Hsp27)單克隆抗體

Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

編號(hào)UMAA693Hu27
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))D2
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA693Hu01-27kDa熱休克蛋白(Hsp27)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate Primary Ab: 3μg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)Hsp27的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別Hsp27。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UEPA693Hu61	27kDa熱休克蛋白(Hsp27)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA693Hu01	27kDa熱休克蛋白(Hsp27)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA693Hu61	27kDa熱休克蛋白(Hsp27)活性蛋白	Cell?culture;?Activity?Assays.
UPAA693Hu01	27kDa熱休克蛋白(Hsp27)多克隆抗體	WB; IHC; ICC/IF
UFAA693Hu01	抗27kDa熱休克蛋白(Hsp27)多克隆抗體	FCM
ULAA693Hu81	27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
ULAA693Hu71	27kDa熱休克蛋白(Hsp27)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
UFAA693Hu81	抗27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記)	FCM
UMAA693Hu22	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; ICC/IF
UMAA693Hu21	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; FCM
UMAA693Hu23	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; FCM
UMAA693Hu24	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC
UMAA693Hu25	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC
UMAA693Hu27	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; ICC/IF
UMAA693Hu29	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; ICC/IF
UFAA693Hu06	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	FCM
UFAA693Hu08	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	FCM
UFAA693Hu04	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	FCM
UFAA693Hu02	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	FCM
ULAA693Hu74	27kDa熱休克蛋白(Hsp27)單克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
USEA693Hu	27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UHEA693Hu	27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA693Hu	27kDa熱休克蛋白(Hsp27)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA693Hu01	27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Animal Science Journal	The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738]
Nature?Communications	HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953]
Advances in Experimental?Medicine?and?Biology	Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
Diabetes Research and Clinical Practice	The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684]
Journal of Immunology Research	Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients []
Journal of Clinical Medicine	Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Medicine	Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771]
Beilstein Archives	Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation []

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