骨鈣素(OC)引物對(duì)
Primer Pair for Osteocalcin (OC)
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
- 編號(hào)UPGA471Ga01
- 物種Chicken (Gallus，雞) 相同的名稱(chēng)，不同的物種。
- 適用片段Ala21~Val97
- 上游引物CGGAATTCGCGCCGGACGGCTCG
- 下游引物CCGCTCGAGTCAGACGGGGCCGTAGAAGC
- 純化方式RPC
- 酶切位點(diǎn)5'-EcoRI, 3'-XhoI
- 解鏈溫度55-60℃
- 性狀凍干粉
- 應(yīng)用PCR
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 1 OD
- 價(jià)格 ¥ 180
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
用法
利用無(wú)菌水稀釋到10-100uM，可渦旋混勻。
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-20°C不超過(guò)12個(gè)月。
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| 編號(hào) | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA471Ga01 | 骨鈣素(OC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA471Ga01 | 骨鈣素(OC)多克隆抗體 | WB |
| UMAA471Ga21 | 骨鈣素(OC)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA471Ga | 骨鈣素(OC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA471Ga | 骨鈣素(OC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPGA471Ga01 | 骨鈣素(OC)引物對(duì) | PCR |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Naunyn-Schmiedeberg's Archives of Pharmacology | Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619] |
| FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352] |
| American Diabetes Association | Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopenia [Pubmed:25633418] |
| International Journal of Nanomedicine | Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation [Pubmed:25678785] |
| Medical Journal fo the Islamic Republic of Iran | Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models [Pubmed:25679008] |
| BioMed Research International | Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathway [Pubmed:25834823] |
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| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb] |
| Bioscience Trends | Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells [PubMed: 26355224] |
| J Nutr Biochem | Body fat loss induced by calcium in co-supplementation with conjugated linoleic acid is associated with increased expression of bone formation genes in adult mice [PubMed: 26454511] |
| Int J Mol Med | Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway [PubMed: 26677102] |
| Experimental And Therapeutic Medicine | Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway [Pubmed:27347050] |
| International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats [profile:303311214] |
| Journal of Alloys and Compounds | Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants [science:S0045653516302016] |
| Bone | The effects of strength training and raloxifene on bone health in aging ovariectomized rats [Pubmed:26812611] |
| Biological Trace Element Research | Effect?of?Sodium?Fluoride?on?Bone?Biomechanical?and?Histomorphometric?Parameters?and on?Insulin?Signalingand?Insulin?Sensitivity?in?Ovariectomized?Rats. [Pubmed:26876375] |
| ANNALS OF ANIMAL SCIENCE | Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87] |
| Annals of Animal Science | Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87] |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity [Pubmed:27085967] |
| Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats [:] | |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561] |
| international journal of molecular sciences | Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338] |
| Applied Surface Science | The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption [S0169433216326861] |
| Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. [pubmed:27908549] |
| Chemico-Biological Interactions | Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway [pubmed:27387537] |
| Biomedicine & Pharmacotherapy | Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective … [S0753332216308484] |
| Experimental and Therapeutic?Medicine | Serum?N-terminal?telopeptide?of?type I collagen?as an?early?marker?of fracture nonunion?in?rabbits. [pubmed:28105092] |
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| Mater Sci Eng C Mater Biol Appl. | Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation. [pubmed:27987727] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| Biological Trace Element Research | Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007] |
| Revista Brasileira de Ciência Avícola | Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285] |
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| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
| Asian Journal of Biomedical and Pharmaceutical Sciences | A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/???2-catenin Signaling Pathways in Ovariectomized Rats [ISSN 2249 – 622X] |
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| Journal of Cellular Physiology | Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation. [pubmed:28621452] |
| Materials Science & Engineering?C-Materials for Biological Applications | BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model [Pubmed:30033316] |
| Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats [10.1016:j.jff.2018.04.048] |
| Journal of Ethnopharmacology | Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model [Pubmed:29705515] |
| Life?Sciences | Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway [Pubmed:29883721] |
| PLoS One | Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588] |
| Royal?Society?of?Chemistry | Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances [c7tb02620j] |
| Molecular?Medicine?Reports | Effects of constrained dynamic loading, CKIP?1 gene knockout and combination stimulations on bone loss caused by mechanical unloading [Pubmed:29956799] |
| Journal of?Ultrasound?in Medicine | A Comparison of 1‐and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study [Pubmed:29781183] |
| Tropical?Animal?Health?and Production | Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism [Pubmed:29931604] |
| American Scientific Publishers | Effects of KLD-12/Recombinant Human Bone Morphogenetic Protein-2 Nanofiber Scaffold on the Osteogenic Activity of Rabbit Bone Marrow Mesenchymal Stem Cells [10.1166:jbt.2018.1719] |
| Journal of Materials Chemistry B | Enhanced osteoinductivity of porous biphasic calcium phosphate ceramic beads with high content of strontium incorporated calcium deficient hydroxyapatite [Doi: 10.1039/C8TB01554F] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats [] |
| Nanoscale | Beta-Cyclodextrin Modified Mesoporous Bioactive Glass Nanoparticles/Silk Fibroin Hybrid Nanofibers as an Implantable Estradiol Delivery System for the Potential?… [Pubmed: 30255905] |
| Carbon | In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells [Doi: 10.1016/j.carbon.2018.10.081] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
| Tropical Journal of Pharmaceutical Research | Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway [] |
| Bone?&?Joint?Research | Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise [] |
| Bone?Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992] |
| ACS?Applied?Materials?&?Interfaces | Osteoinductivity of porous biphasic calcium phosphate ceramic spheres with nanocrystalline and their efficacy in guiding bone regeneration [Pubmed: 30629405] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization [Pubmed: 30689288] |
| Chemico-Biological?Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080] |
| Phytomedicine | Effect and mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesis [Doi: 10.1016/j.phymed.2019.152860] |
| Advanced?Healthcare?Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
| American Journal of Translational?Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 [] |
| Journal of Orthopaedic Surgery and Research | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis [] |
| Toxicol Ind Health | Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed?… [Pubmed: 30983556] |
| Tropical Journal of Pharmaceutical Research | Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats. [] |
| medical science monitor | Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132?… [Pubmed: 30923307] |
| Toxicology and applied pharmacology | Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR/S6K1/S6 signaling pathways [Pubmed: 31051157] |
| Journal of Innovations in Pharmaceutical and Biological Sciences | Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and?… [] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | A dual functional bone‐defect‐filling material with sequential antibacterial and osteoinductive properties for infected bone defect repair [Pubmed: 31173657] |
| Polymers | Use of Polyphenol Tannic Acid to Functionalize Titanium with Strontium for Enhancement of Osteoblast Differentiation and Reduction of Osteoclast Activity [Pubmed: 31362449] |
| Bulletin of Experimental Biology and Medicine | Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft [Pubmed: 31630302] |
| International Journal of Nanomedicine | Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway [Pubmed: 31632013] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| Pharmacology | MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal [Pubmed: 31694033] |
| Brazilian Journal of Medical and Biological Research | Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals [Pubmed: 31826180] |
| Biomedicine & Pharmacotherapy | Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone loss [Pubmed: 31846839] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
| Theranostics | EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis [Pubmed: 32194868] |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy [Pubmed: 32172552] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells [Pubmed: 32064734] |
| CELL BIOCHEMISTRY AND FUNCTION | HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinase [Pubmed: 32567086] |
| Cell Death & Disease | QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism [Pubmed: 32382069] |
| Molecular Medicine Reports | Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3?E1 cells via an autocrine mechanism [Pubmed: 33000198] |
| Food & Function | Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the?… [Pubmed: 33140795] |
| ADVANCED FUNCTIONAL MATERIALS | A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration [] |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene [] |
| 淫羊藿水提物及提取藥渣的抗骨質(zhì)疏松藥效比較研究 [] | |
| Chemical Engineering Journal | Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances [] |
| Surfaces and Interfaces | In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching [] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/|??\catenin pathway [33950533] |
| Biochemical Engineering Journal | Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate [] |
| Br J Pharmacol | Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis [34233019] |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism [34511043] |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780] |
| ACS Appl Mater Interfaces | Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic Nanomedicine [Pubmed:35311248] |
| Molecules | Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells [Pubmed:35684342] |
| Journal of Exercise Science & Fitness | Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young Females [Pubmed:35096081] |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing [] |
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